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. 2016 Aug 1;6:30721. doi: 10.1038/srep30721

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) After DHED treatment of OVX Sprague-Dawley rats (200 μg/kg, p.o.), E₂ concentration increases in the hypothalamus but not in the serum, as measured by LC-MS/MS assay (n = 3–4 rats/time point). Data represent mean ± SEM. (b) Administration of a deuterium-labeled (d₃-) DHED, produces d₃-E₂ (middle trace) in the hypothalamus without influencing endogenous E₂ level (top trace). The chart shows LC-MS/MS analyses from tissue harvested 1 h after d₃-DHED (200 μg/kg, p.o.), using [¹³C₆]E₂ added to the tissue homogenate as an analytical reference.