Skip to main content
. 2016 Aug 1;6:30700. doi: 10.1038/srep30700

Figure 2. The gene of calcium-binding mitochondrial carrier protein is mispredicted in the genome annotation of B. floridae.

Figure 2

(a) The calcium-binding mitochondrial carrier protein 330210_PRF0 of B. belcheri is an ortholog of the B. floridae protein XP_002602990.1, but the latter contains a C-terminal extension with a Peptidase_M2 domain homologous with angiotensin-converting enzymes. Tblastn searches of the database of B. floridae ESTs with the XP_002602990.1 protein identified ESTs (BI386388.1 and BW904813) that indicate that the presence of the Peptidase_M2 domain in XP_002602990.1 reflects an error in gene prediction, permitting the correction of this sequence (XP_002602990.1_corr). (b) Alignment of the sequences of 330210_PRF0, XP_002602990.1 and XP_002602990.1_corr. Residues supported by both ESTs (BI386388.1 and BW904813 are underlined. Note that the apparent difference between the domain architectures of calcium-binding mitochondrial carrier protein orthologs of B. belcheri and B. floridae reflected an error in gene prediction and not true change in gene structure. Color code for Pfam-A domains: EF-hand – green; Mito_carr – red; Peptidase_M2 – blue.