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. 2016 Aug 1;6:30700. doi: 10.1038/srep30700

Figure 5. Evidence that a protein with a novel domain architecture present in the predicted proteomes of B. belcheri and B. floridae reflects errors of gene prediction and not true innnovation.

Figure 5

Protein 018300_PFF0 of B. belcheri and its B. floridae ortholog, XP_002588697.1 contain a Lectin_C domain and five tandem fn2 domains. (a) pictogram of the domain architecture of XP_002588697.1. (b) positions of the various Pfam_A domains in the linear sequence of XP_002588697.1. The co-occurrence of a Lectin_C domain and fn2 domains, however is refuted by ESTs that match the region linking these two domain types. Tblastn searches of the database ESTs with XP_002588697.1 identified several ESTs that match the Lectin_C domain (ESTs BW716515, BW784251 andBW722680) or the fn2 region (BW803184) but not both parts of the chimeric XP_002588697.1 protein (c). The alignment shows only residues 241–480 of XP_002588697.1, containing the Lectin_C domain and the first two fn2 domains. Note that ESTs BW716515, BW784251, BW722680 match just the Lectin_C domain and define the stop codon of the upstream protein containing the Lectin_C domain. Also note that EST BW803184, that matches the fn2 domains, defines the N-terminal secretory signal peptide (residues underlined) of the downstream protein containing fn2 domain(s).