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. 2016 Aug 1;6:30649. doi: 10.1038/srep30649

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Copyright © 2016, The Author(s)

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(A) GLI2, (B) type I collagen and (C) SMA expression in HCV-exposed fibroblasts pre-treated or not with GANT61 61 (5 μM). Lamin B or β-Actin were used as loading controls. (D) Dot-blot analysis showing type I collagen release in the culture conditioned medium of fibroblasts pre-treated or not with Gant 61 and exposed to HCV-containing sera. (E) Expression of LC3-I/II in the presence or in the absence of bafilomycin in fibroblasts pre-treated or not with Gant 61 and exposed to HCV-containing sera. β-Actin was used as loading control and a representative experiment out of 3 is shown. The histograms represent the mean plus SD of the densitometric analysis of the ratio of specific molecules on loading control of 3 different experiments.