Table 3. Human VTA neurons expressing VGluT2 mRNA or TH-immunoreactivity.
| VTA Subdivision | Subject | VGluT2-only | VGluT2-TH | TH-only |
|---|---|---|---|---|
| RLi | Subject 1 | 49.48% (n = 383) | 14.86% (n = 115) | 35.66% (n = 276) |
| Subject 2 | 34.6% (n = 228) | 5.16% (n = 34) | 60.24% (n = 397) | |
| Mean ± SEM | 42.04% ± 7.44% | 10.01% ± 4.85% | 47.95% ± 12.29% | |
| PBP | Subject 1 | 11.47% (n = 53) | 27.92% (n = 129) | 60.61% (n = 280) |
| Subject 2 | 27.43% (n = 234) | 6.8% (n = 58) | 65.77% (n = 561) | |
| Mean ± SEM | 19.45% ± 7.98% | 17.36% ± 10.56% | 63.19% ± 2.58% | |
| VTASUB | Subject 1 | 9.85% (n = 32) | 9.54% (n = 31) | 80.62% (n = 262) |
| Subject 2 | 17.14% (n = 60) | 10% (n = 35) | 72.86% (n = 255) | |
| Mean ± SEM | 13.49% ± 3.65% | 9.77% ± 0.23% | 76.74% ± 3.88% | |
| PN | Subject 1 | 16.76% (n = 63) | 2.66% (n = 10) | 80.59% (n = 303) |
| Subject 2 | 9.8% (n = 20) | 0.49% (n = 1) | 89.71% (n = 183) | |
| Mean ± SEM | 13.28% ± 3.48% | 1.57% ± 1.08% | 85.15% ± 4.56% |
Percent refers to the number of neurons expressing VGluT2 mRNA without TH-immunoreactivity (VGluT2-only neurons), neurons co-expressing VGluT2 mRNA and TH-immunoreactivity (VGluT2-TH neurons), or neurons expressing TH-immunoreactivity without VGluT2 mRNA (TH-only neurons) divided by the total number of labeled neurons identified in each subject and in each subdivision of the VTA. Numbers in parentheses refer to number of counted neurons of each phenotype.