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. 2016 Aug 1;6:30684. doi: 10.1038/srep30684

Figure 3. The TM1 and the proximal post-TM1 domain of ASIC2a are critical regions for surface trafficking of ASIC2.

Figure 3

(a) Schematic diagram of constructed chimeras. (b) Left, representative confocal images of HEK293T cells expressing each chimera with the PM marker, Lyn-mCh. The scale bar represents 5 μm. Right, pH 4.0-induced currents measured from the cells expressing each chimera. Dashed line indicates the zero current level. (c) Pearson’s correlation coefficient between the PM marker and each chimera (mean ± SEM, * p < 0.05, ***p < 0.001, with one-way ANOVA followed by Bonferroni post-hoc test). The number on each bar indicates n for each condition from three independent experiments. (d) Percentage of cells showing each chimera in specific subcellular localizations (mean ± SEM). For each chimera, 250 cells were counted from five independent experiments.