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. 2016 Aug 1;6:30684. doi: 10.1038/srep30684

Figure 8. The N-terminal region of ASIC2a is necessary for the ASIC2a-dependent membrane targeting of ASIC2b.

Figure 8

(a) Co-immunoprecipitation assay in HEK293T cells. GFP-tagged ASIC2 proteins and HA-ASIC2b (left) or GFP-tagged ASIC2b(△N) and Ch3-HA were co-transfected into HEK293T cells, and cell lysates were immunoprecipitated using anti-GFP antibody. The immunoprecipitates were then examined by Western blotting using anti-HA antibody. (b) Schematic diagram depicting heteromeric assembly experiments and representative confocal images of HEK293T cells expressing GFP-tagged ASIC2b in the presence of each ASIC2 protein in pcDNA3.1(+). Line scanning of fluorescent images was processed by using ZEN2011 software (Carl Zeiss). The scale bar represents 5 μm. (c) Percentage of cells showing ASIC2b in specific subcellular localizations in the presence of each ASIC2 protein in pcDNA3.1(+) (mean ± SEM). For each experiment, 250 cells were counted from five independent experiments. (d) Percentage of cells showing N-terminal deleted ASIC2b in specific subcellular localizations in the absence or presence of Ch3 (mean ± SEM). For each experiment, 250 cells were counted from five independent experiments.