Skip to main content
. 2016 Aug 1;6:30654. doi: 10.1038/srep30654

Figure 5. Fluorescence confocal laser scanning microscopy of SK-N-SH cells incubated in the presence of Aβ42 and carboxy-terminal truncated apoE4 forms.

Figure 5

SK-N-SH cells were incubated with 25 ng/ml Aβ42 in the absence (control) or presence of 375 nM lipid-free carboxyl-terminal truncated apoE4 forms for 24 h, as indicated in each panel. Aβ immunostaining of cells was detected with the antibody 6E10 followed by an FITC-conjugated secondary antibody (green). The quantitation of Aβ42 staining in cells based on relative fluorescence intensity measurements is shown in panel f. Values are the means ± SD (n = 4-8). ***p < 0.0001 vs control; AU: arbitrary units. Two images for each experimental condition (i.e. incubation of SK-N-SH cells with 25 ng/ml Aβ42 in the absence (control) or presence of 375 nM lipid-free carboxyl-terminal truncated apoE4 forms for 24 h) showing the Aβ immunostaining of cells are presented in Supplemental Figure 2. In addition, F-actin staining of cells using rhodamine phalloidin is shown in Supplemental Figure 2, to facilitate the visualisation of cells outline, especially in the images with very low Aβ immunostaining.