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. 2016 May 27;8(8):949–966. doi: 10.15252/emmm.201506151

Figure EV3. Impact of Fstl1 on Smad2/3 signaling in infarcted hearts and cultured fibroblasts.

Figure EV3

  1. The expression of p‐Smad2, p‐Smad3, Smad2, and Smad3 in Fstl1‐cfKO and WT hearts was assessed by immunoblotting. Hearts were harvested at 7 days after the surgery. Error bars represent mean ± SEM (n = 3 for each sham group and n = 4 for each MI‐IA group). Statistical analysis was performed by two‐way ANOVA. Post hoc test was performed by Tukey's test.
  2. The effect of recombinant Fstl1 protein on TGF‐β1‐stimulated Smad2/3 signaling pathway in neonatal cardiac fibroblasts (NRCFbs). Recombinant Fstl1 protein (50 ng/ml) or control vehicle was added to serum‐deprived (24 h) NRCFbs at 30 min prior to TGF‐β1 protein (2 ng/ml) stimulation. Samples were harvested at 15 min after TGF‐β1 stimulation. Phosphorylation of Smad 2/3 and Smad 2/3 expression in cell lysates was assessed by immunoblotting. Tubulin was used for internal control. Error bars represent mean ± SEM (n = 3 for each group). Statistical analysis was performed by ordinary one‐way ANOVA and Tukey's test for Smad 2, Kruskal–Wallis test and Dunnett's T3 test for Smad 3. Three independent experiments were performed.

Source data are available online for this figure.