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. 2016 Jul 11;17(8):1184–1203. doi: 10.15252/embr.201541930

Figure EV4. Effect of starvation and exogenous HA‐HuR expression on lung HuR and miRNA levels in BALB/c.

Figure EV4

  1. Scheme of the starvation experiment is shown.
  2. Effect of starvation on lung HuR and let‐7a miRNA levels in the lungs of Starved animals determined by Western blot and qRT–PCR, respectively. Mean C t values for RNA samples isolated from the lungs of Fed and Starved animals were shown (mean ± s.e.m., n = 4). β‐Actin was used as loading control in HuR Western blot.
  3. Scheme of the experiment where HA‐HuR expression or control (pCIneo) plasmids were injected via tail vein.
  4. Effect of HA‐HuR expression plasmid injection on lung HuR level in mouse (mean ± s.e.m., n = 3). Western blot was done on lung lysates obtained from control or HA‐HuR plasmid‐injected animals. β‐Actin was used as loading control. Relative levels of let‐7a miRNA are represented as mean C t values for the same amount of lung RNA used.
Data information: Positions of size markers in protein gels used for respective Western blot analysis are shown against each panel. p.i.: post injection.