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. Author manuscript; available in PMC: 2017 Aug 5.
Published in final edited form as: Vaccine. 2016 Jul 9;34(36):4304–4312. doi: 10.1016/j.vaccine.2016.06.080

Figure 5. Cytokine production in response compound 2, compound 4 and compound 7 from pig or human PBMCs.

Figure 5

A) Structure of compound 7, left, and HEK hTLR7 or hTLR8 cell response to compound 7, right. HEK hTLR7 or hTLR8 cells were subjected to treatment with increasing concentrations of compound 7 followed by determination of relative NF-κB activity versus control using a QuantiBlue colorimetric assay. Graphs represent three separate experiments ± SEM. EC50 in each cell line, outlined under structure, was determined by non-linear curve fitting. B) PBMCs from each indicated species were subjected to treatment with increasing concentrations of compounds 2, 4 or 7 for 24 h followed by assessment of IFNα and TNFα cytokine production from supernatants by ELISA. Human PBMC graphs represent average response from three separate donors ± SEM. Pig PBCM graphs represent average response from two replicates ± SD.