Table 2.
Cross-neutralization of the H5N8 NE14 (A) or H5N6 SC14 (B) virus by monoclonal antibodies measured by plaque reduction assay.
A
| Antibody concentration (µg/ml) |
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Antibody | 20 | 10 | 5 | 2.5 | 1.25 | 0.625 | 0.3125 | 0.15625 | 0 |
| 100F4 | 0 | 0 | 0 | 0 | 0 | 2.7 ± 0.6* | 19.3 ± 2.5 | 59.3 ± 3.5 | 59.0 ± 7.5 |
| 65C6 | 0 | 0 | 0 | 0 | 0 | 8.0 ± 2.0 | 42.7 ± 5.0 | 59.3 ± 5.5 | 59.0 ± 7.5 |
| AVFluIgG01 | 59.3 ± 4.0 | 54.3 ± 3.2 | 53.3 ± 4.2 | 57.0 ± 4.6 | 62.3 ± 4.0 | 56.3 ± 7.4 | 57.0 ± 3.6 | 59.3 ± 6.7 | 59 ± 7.5 |
| AVFluIgG03 | 54.0 ± 6.1 | 54.3 ± 4.0 | 56.7 ± 4.2 | 58.0 ± 6.2 | 59.0 ± 6.6 | 58.3 ± 3.8 | 57.7 ± 2.5 | 61.0 ± 2.6 | 59.0 ± 7.5 |
| FLA5.10 | 0 | 0 | 0 | 0 | 0 | 0 | 1.0 ± 1.0 | 4.7 ± 2.5 | 59.0 ± 7.5 |
| FLD21.140 | 0 | 0 | 0 | 0 | 0 | 0 | 0.7 ± 0.6 | 1.0 ± 1.0 | 59.0 ± 7.5 |
| S139/1 | 63.7 ± 6.0 | 56.0 ± 5.3 | 55.7 ± 4.0 | 60.7 ± 2.1 | 59.7 ± 5.7 | 60.3 ± 1.2 | 64.3 ± 1.5 | 60.7 ± 5.1 | 59.0 ± 7.5 |
| VRC01 |
55.0 ± 4.0 |
56.7 ± 1.5 |
58.7 ± 1.5 |
62.0 ± 6.2 |
61.3 ± 3.1 |
61.7 ± 6.4 |
61.0 ± 4.6 |
63 ± 5.6 |
59.0 ± 7.5 |
| B | |||||||||
| 100F4 | 0 | 0 | 0 | 0 | 1.0 ± 1.7* | 11.0 ± 4.6 | 55.0 ± 4.6 | 60.0 ± 7.9 | 62.0 ± 3.5 |
| 65C6 | 0 | 0 | 0 | 0 | 16.0 ± 3.6 | 15.0 ± 4.4 | 27.0 ± 5.2 | 53.0 ± 3.6 | 62.0 ± 3.5 |
| AVFluIgG01 | 65.0 ± 3.6 | 64.3 ± 6.1 | 61.7 ± 4.2 | 66.0 ± 6.0 | 61.0 ± 6.2 | 59.0 ± 6.2 | 63.0 ± 8.5 | 60.0 ± 9.0 | 62.0 ± 3.5 |
| AVFluIgG03 | 63.0 ± 4.4 | 61.7 ± 3.1 | 60.0 ± 5.2 | 60.0 ± 5.3 | 60.7 ± 5.5 | 64.0 ± 7.5 | 62.0 ± 6.9 | 62.7 ± 3.5 | 62.0 ± 3.5 |
| FLA5.10 | 0 | 0 | 0 | 0 | 9.0 ± 5.2 | 6.0 ± 5.2 | 57.0 ± 3.0 | 54.7 ± 4.9 | 62.0 ± 3.5 |
| FLD21.140 | 0 | 0 | 0 | 0 | 10.0 ± 9.6 | 19.0 ± 6.9 | 26.0 ± 8.7 | 58.3 ± 2.1 | 62.0 ± 3.5 |
| S139/1 | 64.7 ± 4.0 | 65.3 ± 5.5 | 62.3 ± 2.5 | 67.3 ± 4.2 | 59.7 ± 3.8 | 60.7 ± 3.8 | 64.0 ± 6.6 | 63.0 ± 3.6 | 62.0 ± 3.5 |
| VRC01 | 59.7 ± 3.1 | 63.7 ± 2.1 | 61.0 ± 7.5 | 63.3 ± 3.2 | 62.0 ± 2.6 | 61.3 ± 4.2 | 63.7 ± 2.1 | 64.3 ± 5.5 | 62.0 ± 3.5 |
Mean ± SD of the number of plaques in triplicates.