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Sequence comparison between yeast surface display and next-generation sequencing discovered antibodies. (A), (B) VH alignments of BM1, BM3, BM17, SP19 and RAM1.5 and of SP1 and RAM1.4 showed mutations through the complete VH sequences, indicating they are somatic variants. (C), (D) VL alignments of BM1/BM3/BM17, SP19 and RAM1.5 and of SP1 and RAM1.4 also showed mutations. Mutations are indicated by different colors.
