Table 1.
List of the antibodies isolated from the bone marrow and spleen combinatorial libraries by yeast display and abundance of their VH and VL sequences in the corresponding repertoires assessed by next-generation sequencing. Note that BM3 and BM17 have the same CDRH3 sequences, but differ in sequence outside of CDRH3 (Fig. 5).
| Antibody | Source | CDRH3: CDRL3 | Gene usage | VH:VL abundance (percentage and rank) |
|---|---|---|---|---|
| BM1 | bone marrow | CTRSEFVNFGWFAYW: CQQYHNFPRTF | IGHV1-IGHD2-IGHJ3: IGKV4-IGKJ1 |
2.6%, 3rd: 3.1%, 3rd |
| BM3 | bone marrow | CTRSEYVNFGWFAYW: CQQYHNFPRTF | IGHV1-IGHD2-IGHJ3: IGKV4-IGKJ1 |
5.1%, 2nd: 3.1%, 3rd |
| BM17 | bone marrow | CTRSEYVNFGWFAYW: CQQYHNFPRTF | IGHV1-IGHD2-IGHJ3: IGKV4-IGKJ1 |
1.2%, 5th: 3.1%, 3rd |
| SP1 | spleen | CSRDRTWYGTFYAMDYW: CHQYHRSPYTF |
IGHV1-IGHD2-IGHJ4: IGKV4-IGKJ2 |
0.9%,5th: 2.8%,3rd |
| SP19 | spleen | CTRSEFVNFGWFAYW: CHQYHNYPRTF | IGHV1-IGHD2-IGHJ3: IGKV4-IGKJ1 |
5.3%,2nd: 1.2%,4th |