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. 2016 May 21;8(6):1126–1135. doi: 10.1080/19420862.2016.1189050

Figure 2.

Figure 2.

Enrichment of GCGR specific phage after 3 rounds of VLP selections, scFv ELISA and mAb binding to GCGR expressing HEK293 cells. (A) GCGR-specific enrichments after 3 rounds of selection with libraries Fab and scFv 73Vκ and (B) libraries Fab and scFv 73Vλ. (C) GCGR-specific phage enrichments with and without counter selections with VLP-null. (D) scFv from library scFv 73Vλ tested for binding in ELISA with immobilized GCGR-VLP or negative control CXCR4-VLP or (E) immobilized ECD-GCGR. Detected with anti-c-myc and read at OD 450 nm. (F) FACS binding of mAbs to GCGR- (dark blue) or GCGRΔECD- (green) expressing HEK293 cells with and without ECD-GCGR competition (light blue). MOCK was included as a negative control (red). mAbs were detected with anti-human Fc-PE.