Figure 4.

Optimization of method of cell incorporation. (a) Schematic of the two methods of cell incorporation—as single cells versus epithelial spheroids plus single CAF. Comparison of (b) aggregation and (c) cell density in solid surrogates with cells incorporated as preformed 231 cell spheroids with CAF or as single cells (both 231 and CAF) following 7 days growth (n = 4–6 surrogates per condition, unpaired t-test). Comparison of (d) aggregation and (e) cell density in perfused surrogates with cells incorporated as preformed 231 cell spheroids with CAF or as single cells (both 231 and CAF) after growth for 7 days (n = 6 surrogates per condition, unpaired t-test, **p ⩽ 0.01). Data in b–e are the mean and SEM.