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. 2016 Jul 27;8:51–60. doi: 10.1016/j.ymgmr.2016.06.009

Fig. 2.

Fig. 2

Representative of fusion PCC subunits purification and western blot. (a) Reducing SDS-PAGE of a representative PCC subunit lysate is compared with purified one after using nickel affinity column purification method. Gels were stained with InstantBlue™ (Expedeon Ltd., Cambridge, UK). (b) Western blot representative of N-terminal PCCA and C-terminal PCCB original and fusion variants using anti-PCCA chicken polyclonal and anti-PCCB mouse polyclonal as primary antibodies respectively.