Fig. 4.

Fusion PCC subunits co-localization. HeLa cells were treated with CF488A green fluorescent labeled PCCA and PCCB fusion variants (0.1 mg/ml final concentration) for 30 min, washed and incubated with MitoView 633 red fluorescent dye for 30 min before fixation with 4% paraformaldehyde. The cells were analyzed for transduction and co-localization by confocal microscopy. Original magnification is 60 ×. Localization of TAT-MTS-PCCA and PCCB-MTS-TAT subunits (tests) and PCCB-MTS, TAT-PCCA and original PCC subunits (controls) are shown in this figure. Merged photos indicate merging photos of red and green fluorescent filters.