Fig. 6.

Schematic representation of the fusion proteins activity. The in vitro enzymatic activity assay of delivered fusion and original subunits was performed as described in Materials and methods. PCCA or PCCB defective lymphocytes were treated with (a) TAT-MTS-PCCA, TAT-PCCA, PCCA, and (b) PCCB-MTS-TAT, PCCB-TAT, PCCB at final concentration of 0.1 mg/ml for different time periods (5 min–24 h). PCC activity was analyzed in isolated mitochondria lysate immediately after the incubation time using the UPLC-MS/MS analysis [7]. A set of control was performed by using un-treated PCCA defective lymphocytes (PCCA def), PCCB defective lymphocytes (PCCB def) and normal lymphocyte cells (normal). Activity assays were conducted at least three times and the values presented are the mean values ± SD. The enzymatic activity values are presented as [MM-CoA] μmol/l/min.