FIG 5.

Activation of Nrf2 inhibits the LXR-induced lipogenic pathway. Primary mouse hepatocytes were cultivated in the presence of 25 mM glucose with 100 nM insulin and supplemented or not for 24 h with 10 μM T0901317 (T0) and 20 μM sulforaphane (SFN) as indicated. (A) Gene expression was analyzed by qRT-PCR and is given relative to that of 18S. Nqo1 is a transcriptional target of Nrf2. Results are the means ± SEM from three independent experiments. *, P < 0.05 for the effect of sulforaphane compared to control dimethyl sulfoxide (DMSO); ##, P < 0.01; ###, P < 0.005 for the effect of the T0901317 treatment. (B) Activity of a 3× LXRE-luc reporter gene transfected in primary mouse hepatocytes treated or not with 10 μM T0901317 and 20 μM sulforaphane as indicated. ***, P < 0.005.