FIG 5.

TAZ stabilization with PCBP2 depletion. (A) Equalization of TAZ levels in control and PCBP2-depleted cells by proteasome inhibition. Cells were treated with siRNAs for 48 h, followed by DMSO or bortezomib for 24 h. TAZ levels were examined by immunoblotting (left) and quantified (right). (B) Prolonged half-life of TAZ with PCBP2 depletion. Cells were treated with siRNAs, followed by cycloheximide (Chx) for the indicated times. The decay of TAZ was measured by immunoblotting (left) and quantified (right). All experiments were replicated three or four times. The error bars indicate SEM. *, P < 0.002.