FIG 8.

Requirement for both Mst1 and Mst2 for PCBP2-mediated changes in TAZ. (A) Individual contributions of both Mst1 and Mst2 to TAZ regulation. MCF10A cells were treated with control or Mst1- or Mst2-specific siRNA and then analyzed by Western blotting for TAZ and GAPDH. (B) Similar effects on TAZ expression with PCBP2 and Mst1/2 depletion. Cells were treated with the indicated siRNAs and analyzed by Western blotting (left) with quantification (right). All experiments were replicated three times. The error bars indicate SEM. *, P = 0.005; ns, not significant. (C) Lack of effect of Lats1/2 depletion on TAZ levels. Cells were treated with the indicated siRNAs and analyzed by Western blotting.