TABLE 3.
Hippo components detected by mass spectrometry in cells expressing Flag-PCBP2a
| Protein | No. of spectra/no. of unique peptides in: |
|||
|---|---|---|---|---|
| Dfo-treated cells |
Fe-treated cells |
|||
| Treatment 1 | Treatment 2 | Treatment 1 | Treatment 2 | |
| PCBP2 | 1,810/45 | 602/26 | 1,305/37 | 1,436/25 |
| PCBP1 | 581/30 | 239/18 | 307/24 | 780/26 |
| Sav1 | 77/24 | 13/8 | 66/25 | 13/3 |
| Mst1 | 67/32 | 56/16 | 62/29 | 67/16 |
| MST2 | 84/39 | 75/20 | 95/41 | 67/14 |
| Ferritin H | 0 | 0 | 3/2 | 0 |
| Ferritin L | 0 | 0 | 2/2 | 0 |
a
Treatment 1, immunoprecipitation with RNase A followed by low-salt wash; treatment 2, immunoprecipitation without RNase A followed by high-salt wash.