TABLE 4.
Hippo components detected by mass spectrometry in cells expressing Flag-PCBP1a
| Protein | No. of spectra/no. of unique peptides in: |
|||||
|---|---|---|---|---|---|---|
| Dfo-treated cells |
Fe-treated cells |
|||||
| Treatment 1 | Treatment 2 | Treatment 3 | Treatment 1 | Treatment 2 | Treatment 3 | |
| PCBP1 | 2,561/52 | 2,784/52 | 2,377/33 | 1,993/42 | 2,800/54 | 3,061/43 |
| PCBP2 | 331/16 | 520/15 | 682/15 | 248/11 | 436/17 | 904/15 |
| Sav1 | 2/2 | 0 | 0 | 3/3 | 0 | 0 |
| Mst1 | 0 | 0 | 0 | 4/3 | 0 | 0 |
| Mst2 | 0 | 0 | 0 | 4/3 | 0 | 0 |
| Ferritin H | 0 | 0 | 0 | 3/3 | 9/6 | 5/2 |
| Ferritin L | 0 | 0 | 0 | 3/2 | 3/3 | 0 |
a
Treatment 1, immunopreciptation without RNase A followed by low-salt wash; treatment 2, immunoprecipitation with RNase A followed by low-salt wash; treatment 3, immunoprecipitation without RNase A followed by high-salt wash.