TABLE 5.
Protein | No. of spectra/no. of unique peptides with: |
||||||
---|---|---|---|---|---|---|---|
Empty vector in: |
Flag | ||||||
Dfo-treated cells |
Fe-treated cells |
||||||
Treatment 1 | Treatment 2 | Treatment 3 | Treatment 1 | Treatment 2 | Treatment 3 | ||
PCBP2 | 0 | 18/7 | 10/7 | 21/7 | 16/9 | 0 | 0 |
PCBP1 | 38/13 | 27/20 | 0 | 36/13 | 25/16 | 12/6 | 0 |
Sav1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mst1 | 7/6 | 0 | 0 | 0 | 0 | 0 | 8/4 |
Mst2 | 6/5 | 0 | 0 | 0 | 0 | 0 | 0 |
Ferritin H | 0 | 0 | 0 | 0 | 2/2 | 0 | 0 |
Ferritin L | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Treatment 1, immunopreciptation without RNase A followed by low-salt wash; treatment 2, immunoprecipitation with RNase A followed by low-salt wash; treatment 3, immunoprecipitation without RNase A followed by high-salt wash.