FIG 4.

Sequential oligonucleotide pulldown assays. (A to F) Detection of oligo-DNA probes containing Single-GATA (A, D), Pal-GATA (B, E), and Tandem-GATA (C, F), which bind to the respective GATA1 proteins, after the first (A to C) and the second (D to F) pulldowns. Filled and open arrowheads indicate oligo-DNA probes bound to GATA1 protein and free oligo-DNA probes, respectively. P, pulldown; I, input. (G to I) Schematic diagrams of predicted binding models of GATA1 to Single-GATA (G), Pal-GATA (H), and Tandem-GATA (I).