FIG 7.

Transient reporter/effector cotransfection/transactivation assays measuring the effect of GATA1 on endogenous Pal-GATA. (A) Schema of the reporter constructs. Pal-GATA sequences in the Lmo2 and Uros gene enhancers are depicted. (B, C) Luciferase activity driven by indicated amounts of G1^WT and G1^R216Q for reporter constructs carrying Lmo2 (B) and Uros gene enhancer (C). Fold increase in luciferase activity was determined relative to the value obtained by samples not transfected with the G1^WT/G1^R216Q expression plasmid. Shown are representative data from two and three independent experiments utilizing reporter constructs containing Lmo2 and Uros gene enhancers, respectively. Means ± standard deviations (SD) from triplicate cultures are shown.