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. 2016 May 12;12(8):1240–1258. doi: 10.1080/15548627.2016.1179405

Figure 5.

Figure 5.

TFE3 and TFEB are required for cytokine production and secretion. (A) Cytokine array blots for control and Tfe3 Tfeb DKO RAW 264.7 cells that were stimulated with LPS for 24 h. Cytokine and chemokine levels of the supernatant were detected using the Mouse Cytokine Array Assay kit (R&D Systems) following manufacturer's protocol. (B) Quantification of cytokine and chemokine levels (n = 3). (Cto F) Relative Quantitative Real-Time PCR analysis of (C) Il1b, (D) Il6, (E) Tnf, and (F) Ccl5 mRNA transcript levels in control, Tfe3 KO, Tfeb KO, Tfe3 Tfeb DKO RAW cell lines after 6, 24, and 48 h of LPS stimulation (n = 3). The P values were calculated by the 2-tailed Student t test analysis of each mutant cell line vs. the control cells. **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001.