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. 2016 Jun 23;12(8):1390–1403. doi: 10.1080/15548627.2016.1184799

Figure 3.

Figure 3.

Inflammasome activation in vivo. (A) The number of CLS in white adipose tissue of 20-wk-old mice was counted as described in Materials and Methods (n = 4 each) (right). Representative H&E stained sections of white adipose tissue are shown (left, scale bar: 100 μm). (B) Total mRNA was extracted from the SVF of white adipose tissue from 20-wk-old mice, and quantitative RT-PCR was done using primers specific for Tnf, Il6, pro-Il1b and Adgre1 (n = 3 each). (C) Tissue lysate of the SVF was subjected to western blot analysis using anti-IL1B and -CASP1 Abs. (D) Serum IL1B level was determined using a commercial ELISA kit (n = 3 to 15). *, P < 0.05; **, P < 0.01; ***, P < 0.001.