Figure 4.

Treg cells in white adipose tissue. (A) Cells in the SVF of white adipose tissue from 20-wk-old mice were incubated with a mixture of anti-CD3E, -CD4, and -IL2RA Abs, and then with -FOXP3 Ab as described in Materials and Methods. FOXP3 and CD4 expression was examined by flow cytometry gated on CD3E⁺ CD4⁺ cells. The proportions of FOXP3⁺ cells among CD3E⁺ CD4⁺ cells (lower left) and the numbers of FOXP3⁺ CD3E⁺ CD4⁺ cells/gm adipose tissue (lower right) (n = 3 each). Representative FACS scattergrams are shown (upper). (B) Cells were incubated with Abs as in (A), and FOXP3 and IL2RA expression was examined by flow cytometry gated on CD3E⁺ CD4⁺ cells. The proportions of FOXP3⁺ IL2RA⁺ cells among CD3E⁺ CD4⁺ cells (lower left) and the numbers of FOXP3⁺ IL2RA⁺ CD3E⁺ CD4⁺ cells/gm adipose tissue (lower right) (n = 3 each). Representative FACS scattergrams are shown (upper). *, P < 0.05; **, P < 0.01.