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. 2016 Jun 23;12(8):1272–1291. doi: 10.1080/15548627.2016.1183081

Figure 2.

Figure 2.

Inhibition of NOS2-mediated NO production decreases SESN2 expression, and increased CASP1 activation. (A-C) BMDMs were primed with LPS (0, 6, 12 h) in the presence or absence of L-NAME followed by the presence or absence of ATP treatment. (A) NO production, (B) immunoblot analysis of the indicated proteins and (C) production of IL1B, IL18 and TNF as measured by ELISA. (D-F) Nos2+/+ and nos2−/− BMDMs were primed with LPS (12 h) followed by ATP treatment. (D) NO production, (E) immunoblot analysis of the indicated proteins and (F) production of IL1B and IL18 as measured by ELISA. Data shown are representative of 3 independent experiments and are the mean ± s .e.m. ***, p < 0.005 from an ANOVA followed by Tukey's post hoc test.