Figure 9.

Human SESN2 is highly expressed in monocytes of septic shock patients. (A-D) Human blood samples were collected from normal subjects (n = 8) and septic shock patients (n = 8). (A) Plasma IL1B and IL18 as measured by ELISA, (B) flow cytometry of isolated human monocytes from blood stained with anti-CD14-FITC antibody to evaluate purity, (C) transmission electron microscopy of morphological changes in mitochondria in isolated human peripheral blood monocytes (arrowhead, damaged mitochondria; red ‘N’, nucleus). Scale bar, 1µm, and (D) immunoblot analysis for SESN2 and Coomassie Blue staining as a control for equal loading of isolated human monocytes. Each symbol represents an individual subject. Data shown are representative of 2 independent experiments and p values from an unpaired Student t test.