Figure 6.
MAPK8 is required for ISO inhibition of anchorage-independent growth in UMUC3 cells and SESN2 was downregulated in human bladder cancer tissues. (A) Representative images of colonies of UMUC3 (shMAPK8) and UMUC3 (Nonsense) cells in a soft-agar assay in the absence or presence of various concentrations of ISO were captured using a microscope. (B) The colony formation was counted under a microscope with more than 32 cells of each colony, and the results presented as colonies/104 cells. The bars indicate mean ± SD from 3 independent experiments. The symbol (*) shows a significant decrease from the vehicle control (p < 0.05). (C) Clinically freshly collected human bladder cancer samples in comparison with the paired adjacent normal bladder tissue were subjected to western blotting to analyze SESN2 expression. N, normal; T, tumor. (D) The proposed model for ISO induction of SESN2 and autophagy and inhibitory effect on human bladder cancer cells.