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. Author manuscript; available in PMC: 2016 Aug 16.
Published in final edited form as: Sci Signal. 2016 Feb 16;9(415):ra18. doi: 10.1126/scisignal.aac4374

Fig. 1. ONC201’s broad-spectrum activity can be attributed to its anti-proliferative and pro-apoptotic effects.

Fig. 1

(A) EC50 of ONC201 was assessed by Cell Titer Glo assay in 23 cell lines treated with ONC201 for 72 hours. (B) Apoptosis measured by Sub-G1 analysis in cells treated with ONC201 for 72 hours. (C) Cell cycle profile analyses in HCT 116 and A549 cells treated with ONC201 for 24 hours. Data is representative of two biological replicates. (D) BrdU incorporation assays in HCT116 and A549 cells treated with ONC201 for 48 hours. (E) Viable cell counts assessed by trypan blue exclusion in cells treated with ONC201 for up to 72 hours. *p < 0.05, Student’s t-test with Holm-Sidak correction. (F) Proliferative capacity of cells after 72 hours in the presence of ONC201, assessed by clonogenic assays. Bars that are not visible indicate a proliferative fraction of 0. Concentrations of ONC201 used for each cell line are listed in table S1. Data in (A), (B), (D), (E) and (F) are means ± SEM from three biological replicates.