Figure 4.

Mouse Fluorotron measurements of known amounts of fluorescein. The accuracy of the Mouse Fluorotron to detect the fluorescein concentration and the change in concentration in a mouse eye was determined by simulating the thickness of a mouse cornea (100 μm) and the depth of the anterior chamber (500 μm) using two cuvettes. The Mouse Fluorotron measured fluorescein concentration in both cuvettes at 2% above actual concentration. A linear fit yielded correlation values of > 0.999 for both cuvettes. Dashed line represents slope = 1.