Effect of timolol plus anesthesia on aqueous flow measured by fluorophotometry. Timolol was applied topically to the corneas of mice compared to those with no topical treatment (Baseline). One hour later, mice were anesthetized using 2,2,2-tribromoethanol (blue bars), ketamine alone (red bars), or ketamine/xylazine combination (green bars). There was a difference in aqueous flow (Fa) amongst anesthesia and treatment groups in general (P < 0.0001). †P < 0.05 vs. 2,2,2-tribromoethanol alone. *P < 0.05 versus ketamine alone.