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. 2016 Jul 6;3(7):160106. doi: 10.1098/rsos.160106

Table 3.

Amounts of adenine and guanine nucleotides in M. blollopis SK-4 and TKG1-2 at −3°C and 10°C. Adenylate energy charge (AEC) and guanylate energy charge (GEC) were calculated according to this formula: ACE (GEC) = {[ATP (DTP)] + [ADP (DDP)]×0.5}/{[ATP (DTP)] + [ADP (DDP)]}+[AMP (DMP)]}. N.D., not detectable.

M. blollopis SK-4
M. blollopis TKG1-2
control −3°C
10°C
control −3°C
10°C
0 h 24 h 48 h 72 h 24 h 48 h 72 h 0 h 24 h 72 h 24 h 72 h
ATP (pmol/OD600 ml) 40 53 83 84 96 98 65 38 28 54 32 33
ADP (pmol/OD600 ml) 4.8 12 33 42 25 14 15 24 18 35 24 31
AMP (pmol/OD600 ml) 1.6 3.1 12 19 7.5 6.2 6.1 12 7.8 14 14 23
GTP (pmol/OD600 ml) 7.7 9.2 18 16 24 22 8.2 6.0 3.7 11 5.5 6.9
GDP (pmol/OD600 ml) 2.5 2.8 7.0 8.0 6.7 5.6 4.1 3.0 2.4 5.3 3.4 3.9
GMP (pmol/OD600 ml) N.D. N.D. 1.4 2.9 1.2 1.6 N.D. N.D. 1.1 2.1 2.3 3.4
ACE 0.91 0.86 0.78 0.72 0.84 0.89 0.84 0.68 0.69 0.69 0.63 0.56
GCE 0.88 0.88 0.81 0.74 0.86 0.85 0.83 0.83 0.68 0.74 0.64 0.62