TABLE 5.
ImageJ-derived band intensities of RT-PCRs of the pqq gene clustera
| Growth condition | FA |
AB |
BC |
CD |
DE |
EG |
16S rRNA |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| BI | RI | BI | RI | BI | RI | BI | RI | BI | RI | BI | RI | BI | RI | |
| LB medium | 0.00 | 0.00 | 3,545.64 | 1.00 | 5,948.40 | 1.00 | 4,618.40 | 1.00 | 5,853.08 | 1.00 | 5,321.52 | 1.00 | 11,781.90 | 1.00 |
| Glucose | 0.00 | 0.00 | 3,257.93 | 0.90 | 3,529.74 | 0.58 | 2,933.79 | 0.62 | 1,057.36 | 0.18 | 5,333.21 | 0.98 | 12,007.78 | 1.02 |
| Glycerol | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | 773.11 | 0.16 | 535.28 | 0.09 | 1,953.48 | 0.35 | 12,237.37 | 1.04 |
| Citrate | 0.00 | 0.00 | 1,217.55 | 0.35 | 0.00 | 0.00 | 1,577.18 | 0.35 | 1,044.11 | 0.18 | 3,111.60 | 0.60 | 11,494.00 | 0.98 |
a
Intergenic regions between pqqF and pqqA, pqqA and pqqB, pqqB and pqqC, pqqC and pqqD, pqqD and pqqE, and pqqE and pqqG are represented as FA, AB, BC, CD, DE, and EG, respectively. Relative intensity (RI) is calculated from the normalizing band intensity (BI) of the pqq PCR (target) to the PCR of the 16S rRNA gene (reference), with the LB medium growth condition serving as the control treatment.