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Phosphatase expression and secretion. Spotting of recombinant D. radiodurans (A) and E. coli (B) cells (10 μl; OD600, ∼1) to assess expression and activity of phosphatases, or of spent medium (10 μl) to identify extracellular secretion of PhoK in D. radiodurans (C) and E. coli (D) on modified histochemical plate containing PDP-MG (pH 7.0), was performed.
