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. Author manuscript; available in PMC: 2017 Jan 27.
Published in final edited form as: Nature. 2016 Jul 27;535(7613):551–555. doi: 10.1038/nature18928

Extended Data Figure 4. Summary of experiment on Figure 2c.

Extended Data Figure 4

a, We repeated the experiment in Fig. 2c with n=4 independent primary cultures per group. Similar results were obtained. The extracellular mitochondria-depleted astrocyte media (mdACM) group was significantly different compared to the ACM group. Furthermore, in this repeated experiment, there was also statistical significance between controls (OGD-damaged neurons alone) versus those treated with mitochondria-containing astrocyte media (ACM), and there was no statistically significant worsening when comparing control versus mitochondria-depleted groups (mdACM). Taken together, these two separate experiments suggest a modest but statistically significant neuroprotection induced by astrocyte-derived mitochondria. All values are mean +/− SEM. b, Mitotracker Red CMXRos (200 nM) was incubated without astrocytes to obtain no-cell-derived media (negative control). Media was collected and further incubated with neurons following oxygen-glucose deprivation. After 24 hours, there was no mitochondrial signal observed. Scale: 100 μm.