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Dendritic cell (DC) uptake of M-HSP70-PCs. DCs were pulsed with M-HSP70-PCs at 37°C for 12 h. The cells were then incubated with specific monoclonal antibodies against Melan-A and NY-ESO-1, and visualized by fluorescence microscopy. Melan-A (red) and NY-ESO-1 (green) proteins were observed on the DC surface. (DCs from patient no. 4 were used).
