Table 1.

Primers used to detect PVY-O, PVA, and PLRV

Primer pairs	Sequence	PCR type
PLRV-qPCR F	5′-CAACAACCAAGAAGGCGAAG-3′	qPCR
PLRV-qPCR R	5′-ACCATAACCACTGGCTGAACTC-3′
PLRV-186F	5′-GAGTCTATCAGACTGTCCGGCATT-3′	RT-PCR
PLRV-4391R	5′-ATTGAGAGTTAATACTCAGAG-3′
PVA-807F	5′-CCGAAACTCTTGATGCAAGCGAA-3′
PVA-807R	5′-CACCCCCTTCACGCCTAAAAGGTG-3′
PVY CP-801F	5′-GCAAATGACACAATTGATGCA-3′
PVY CP-801R	5′-CATGTTCTTGACTCCAAGTAGAGTA-3′

PVY-O, Potato virus Y-O; PVA, Potato virus A; PLRV, Potato leafroll virus.

Both PVY-O and PVA were amplified using the reverse transcription-polymerase chain reaction (RT-PCR), and absolute quantification of PLRV copies was conducted by real-time RT-PCR (qRT-PCR).