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A mutation in algO/prc increases activity of the algU promoter
| Strain | Relevant Genotype | Alginate Phenotype |
β-galactosidasea (Miller Units) |
|---|---|---|---|
| DH5α | E. coli (PalgT/U-lacZ) | Alg− | 11 ± 12 |
| PAO1 | Wild-type (PalgT/U-lacZ) | Alg− | 179 ± 45 |
| PDO300 | PAO mucA22 (PalgT/U-lacZ) | Alg+ | 520 ± 62 |
| RTS22 | PAO mucA22 sap22 (PalgT/U-lacZ) | Alg− | 855 ± 65 |
Mean β-galactosidase activity (± standard deviation) expressed in Miller units (Miller, 1972) from at least three independent cultures.
