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UHPLC conditions for the target metabolites.
| Sample | Water (%) |
ACN (%) |
Wavelength (nm) |
Flow rate (mL/min) |
Retention time (min) |
|---|---|---|---|---|---|
| Rutin | 90 | 10 | 350/254 | 0.4 | 4.9 |
| Quercetin | 85 | 15 | 350/254 | 0.4 | 6.9 |
| Kaempferol | 80 | 20 | 350/254 | 0.4 | 5.4 |
| Daidzein | 85 | 15 | 300/254 | 0.4 | 4.6 |
