Table 3.

Product pattern of ALOX15 mutants

Species (mutant)	Relative product share, %
	15-HETE	12-HETE
Human (WT)	78.7 ± 1.6	21.3 ± 1.6
Human (Met419Thr)	34.7 ± 8.7	65.3 ± 8.7
Human (Thr594Val)	81.5 ± 1.2	18.6 ± 0.9
Gibbon (WT)	46.1 ± 5.2	53.9 ± 5.2
Gibbon (Ile418Ala)	<1	99 ± 14
Gibbon (Ile418Phe)	83.6 ± 3.5	16.4 ± 3.5

Human and gibbon ALOX15 orthologs were expressed in E. coli. The product pattern was analyzed by consecutive RP-HPLC, SP-HPLC, and chiral-phase HPLC. Four independent expression experiments were performed, and each sample was quantified twice. Means ± SD of the relative shares of the two major reaction products are given.