Fig. S1.

Schematic representation of reporter mice and lympho-proliferation of FoxP3-GFPcre^KI/Y PD-1 KO mice. (A) Map of the FoxP3-GFPcre locus is shown. Filled boxes, exons; gray box, 3′UTR of Foxp3 gene; I, IRES. (B) Principle of Cre-Lox–mediated tdRFP. (C) The 3- to ∼11-wk-old male FoxP3-GFPcre^KI/Y PD-1 WT or PD-1 KO mice were analyzed for serum lipase concentration (excluding the maximum and minimum values). (D) Total number of mononuclear cells in the pancreas, pancreatic LNs, SPL, and LNs of 3- to ∼10-wk-old FoxP3-GFPcre^KI/Y PD-1 WT or PD-1 KO mice. (E) The number of CD4⁺ and CD8⁺ T cells from the pancreas and pancreatic LNs and the number of CD44⁺ in CD4⁺ or CD8⁺ T cells from the pancreatic LNs of mice are as in D. (F) The number of CD4⁺, CD8⁺, CD44⁺CD4⁺, CD44⁺CD8⁺, and FoxP3⁺(GFP⁺) T cells from the SPL of mice as in D. (G) LN cells were analyzed for the frequency and number of CD4⁺, CD8⁺, CD44⁺CD4⁺, CD44⁺CD8⁺, and FoxP3⁺ (GFP⁺) T cells and for the ratio of CD44⁺ (gated on CD4⁺ or CD8⁺ T cells) and FoxP3⁺ (GFP⁺) T cells of mice as in D. Data are representative of 3 to ∼7 mice per group and shown as the means (±SEM). *P < 0.05, **P < 0.01, ***P < 0.001.