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Base pairs involved in NN parameters considered in this study. (A) Canonical pairs adenosine–uracil and guanosine–cytidine, (B) guanosine–uracil wobble pair, (C) nonnatural isoguanosine–isocytidine, (D) nonnatural 2,6-diaminopurine–uracil, and (E) inosine–cytidine.