Fig. 2.

Stable maintenance of both cell types facilitates growth in sucrose + cycloheximide media. (A) Representative time courses of the fraction of somatic cells in cultures of the unicellular differentiating strain (yMEW192 and yMEW192 convertant) initiated at various cell type ratios and passaged in YPD containing 10 nM β-estradiol and 600 nM cycloheximide. (B) The steady-state fraction of somatic cells was determined as in A for various cycloheximide and β-estradiol concentrations. Error bars represent 2 standard deviations, calculated using data obtained from three biological replicates. (C) Dependence of growth rate on fraction of somatic cells in cultures of the unicellular differentiating strain growing in 0.5% sucrose minimal media containing 150 nM cycloheximide and β-estradiol concentrations of 0, 0.3,1, 3, 10, and 50 nM, respectively. Error bars represent 95% CIs of the mean, determined using data obtained from three biological replicates.