Dynamics of memory and recalled γδ Trm in the MLN. (A) Superimposed tracks of γδ T cells from movies taken at different time points normalized to their starting coordinates. Magenta, 30 dpi (memory); blue, 0 dpi (naïve); green, 1 dpr; and red, 2 dpr. (B) Track length. (C) Mean track speed. (D) Straightness data were obtained from 18 movies (naïve = 3, memory = 5, 1 dpr = 5, and 2 dpr = 5). Each dot represents one cell and data are expressed as mean ± SEM and analyzed by one-way ANOVA and Dunn’s multicomparison test; ***P < 0.001.