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. 2016 Jul 12;113(30):E4415–E4422. doi: 10.1073/pnas.1607074113

Fig. 2.

Fig. 2.

Nuclear UVR8 regulates UV-B responses. (A and B) Immunoblot analyses: (A) UVR8, GR-UVR8, and actin (control) proteins in 4-d-old uvr8-7, wild-type (Ws), uvr8-7/Pro35S:GR-UVR8 (uvr8-7/GR-UVR8 1 and 6), and uvr8-7/Pro35S:GR-UVR8W285F (uvr8-7/GR-UVR8W285F 4 and 8) lines. (B) UVR8 and GR-UVR8 homodimers and monomers in nonheat-denatured protein extracts of 4-d-old uvr8-7, wild-type (Ws), and uvr8-7/Pro35S:GR-UVR8 line 6 seedlings. The latter seedlings were treated with 10 µM dexamethasone (Dex) or ethanol (mock). Seedlings were irradiated for 15 min with (+) or without (−) broadband UV-B. Actin was the loading control. (C) Hypocotyl growth in weak white light with or without narrowband UV-B and treated with 10 µM dexamethasone (Dex) or ethanol (EtOH) or no treatment (−). Images of representative individuals (Upper) and hypocotyl lengths (Lower) of 4-d-old seedlings; means with SE; n = 15. (D) Quantitative RT-PCR analysis of HY5 mRNA in 7-d-old seedlings incubated for 3 h in one-half-strength MS (Ctrl.) with 10 µM dexamethasone (Dex) or ethanol (EtOH) before irradiation for 2 h with narrowband UV-B (+) or without (−). Data for Col with ethanol treatment were set to 1; means with SE; n = 3.